Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.
Mini-PROTEAN TGX Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS.
Mini-PROTEAN TGX Precast Gels do not contain SDS. 1-D polyacrylamide gel electrophoresis for protein separation.Uses same buffers and band patterns as Laemmli systemsĪpplications and Uses of Mini-PROTEAN TGX Precast Gels.Bottom-open cassette design allows easy gel handling and faster blotting setup.Inexpensive buffer system means low running costs.Numbered, marked well outlines on the cassette ease sample loading and identification.Electrophoresis run times in as short as 15 min.These gels can also be used in the discontinued Mini-PROTEAN 3 Cell.įeatures and Benefits of Mini-PROTEAN TGX Precast Gels Mini-PROTEAN Precast Gels are compatible with Mini-PROTEAN Tetra (1–4 gels) and Mini-PROTEAN ® Dodeca™ (1–12 gels) Cells. Mini-PROTEAN Tetra Cells with Protein Blotting ModulesĪll blotting modules include modular electrode assembly, gel holder cassettes, fiber pads, and Bio-Ice cooling unit.Long shelf life Mini-PROTEAN ® TGX™ Precast Gels are an innovative PAGE system designed to provide very fast separation while maintaining high resolution in standard Tris-glycine buffer. Mini-PROTEAN Tetra Cells with PowerPac Power Supplies Gel Compatibility Applications include oligonucleotide purity analysis, RNase protection assays, and northern blotting. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands. To configure your own electrophoresis cell, order the 4-gel Mini-PROTEAN Tetra cell ( 1658004) and select a casting module from the Mini-PROTEAN Tetra Cell Accessories tab. Mini-PROTEAN TBE-Urea precast gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Mini-PROTEAN Tetra Cells Gel Compatibility Any 2-gel Tetra Cell can be converted to a 4-gel system by adding a companion running module ( 1658038). The Mini-PROTEAN Tetra Cell is configurable for 1–4 precast or handcast gels in different thicknesses, and bundled with power supplies and blotting modules. Tetra Cells for hand casting include casting frames and stands, glass plates, 10-well gel combs, sample loading guide, and TGX Stain-Free FastCast Acrylamide, 10% ( 161-0182).All Tetra Cells include buffer tank, electrode assembly, lid with power cables, and mini cell buffer dam.Evaluating sample preparation conditions.Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE).Side-by-side casting stand** allows access to both gels simultaneously, and a spring-loaded lever creates a tight seal against the thermoplastic rubber gasket.Casting frames** with simple cam closure provide precision alignment on any flat surface.Thermoplastic casting gaskets seal tightly to ensure leak-free casting.Can be used for western blotting with the Mini Trans-Blot Module.Can run one to four precast or handcast mini gels in 15–20 min.The Mini-PROTEAN Tetra Cell eliminates tedious assembly procedures and has a patented sealing mechanism* that prevents assembly errors during gel casting. The versatile, easy-to-use Mini-PROTEAN Tetra Cell is a vertical mini gel electrophoresis system designed for fast, simple setup, facilitating rapid sample optimization, method development, and targeted protein analysis.
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